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32884

Sigma-Aldrich

Giemsa Stain, Modified Solution

according to Giemsa

Sinônimo(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

Número CAS:
51811-82-6
Número MDL:
MFCD00081642
Código UNSPSC:
12171500
ID de substância PubChem:
329754377
NACRES:
MA.02

forma

liquid

Nível de qualidade

200

qualidade

according to Giemsa

técnica(s)

UV/Vis spectroscopy: suitable

cor

dark blue

pH

6.9

densidade

1.036 g/cm3 at 20 °C

adequação

passes test for UV spectrum

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

chave InChI

NALREUIWICQLPS-UHFFFAOYSA-N

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Descrição geral

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Aplicação

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Outras notas

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Princípio

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Órgãos-alvo

Eyes,Central nervous system

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

51.8 °F - closed cup

Ponto de fulgor (°C)

11 °C - closed cup

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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S R Patil et al.
Science (New York, N.Y.), 173(3999), 821-822 (1971-08-27)
Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the
Xiao Liu et al.
EBioMedicine, 46, 42-53 (2019-08-10)
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Therese Wilhelm et al.
Nature communications, 10(1), 3585-3585 (2019-08-10)
Replication stress, a hallmark of cancerous and pre-cancerous lesions, is linked to structural chromosomal aberrations. Recent studies demonstrated that it could also lead to numerical chromosomal instability (CIN). The mechanism, however, remains elusive. Here, we show that inducing replication stress
Young-Whan Choi et al.
Journal of ethnopharmacology, 178, 17-24 (2015-12-18)
The giant butterbur Petasites japonicus is used to treat asthma and allergic diseases in traditional Korean, Japanese, and Chinese medicine. To elucidate the anti-allergic effect of Petasites genus, we studied effects of several compounds from Petasites japonicus leaves and found
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4-Hydroxy-2-(4-hydroxyphenethyl)isoindoline-1,3-dione (PD1) is a synthetic phthalimide derivative of a marine compound. PD1 has peroxisome proliferator-activated receptor (PPAR) γ agonistic and anti-inflammatory effects. This study aimed to investigate the effect of PD1 on allergic asthma using rat basophilic leukemia (RBL)-2H3 mast
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