Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

GE28-9852-54

rProtein A GraviTrap

Cytiva 28-9852-54, pack of 10 × 1 mL

Sinônimo(s):

GraviTrap

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

forma

prepacked column

reticulação

cross-linked

embalagem

pack of 10 × 1 mL

fabricante/nome comercial

Cytiva 28-9852-54

condição de armazenamento

(20% Ethanol)

técnica(s)

liquid chromatography (LC): suitable

volume do leito

1 mL

matriz

(Highly cross-linked agarose, 4%)

Grupo ativo da matriz

Protein A functional group

tamanho de partícula

(90 µm)

capacidade

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

técnica de separação

affinity

temperatura de armazenamento

2-8°C

Descrição geral

rProtein A GraviTrap includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose Fast Flow.

Aplicação

rProtein A GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Características e benefícios

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap ~ 50 mg human IgG/column

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 3

1 of 3

PD Miditrap G-25 Cytiva 28-9180-08

GE28-9180-08

PD Miditrap G-25

Column PD-10, Empty Cytiva 17-0435-01

GE17-0435-01

Column PD-10, Empty

Artigos

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocolos

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica