G25150
Sephadex® G-25
Medium
Sinônimo(s):
Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins
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About This Item
Produtos recomendados
Nível de qualidade
técnica(s)
buffer exchange: suitable
Grupo ativo da matriz
phase
inchaço
1 g swells to 4-6 mL
tamanho da esfera
50-150 μm
aplicação(ões)
life science and biopharma
compatibilidade
Cytiva
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
chave InChI
BCPVZFFJGCURNR-OCOFDJSDSA-N
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Descrição geral
Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.
Aplicação
Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
- in the desalting of protease
- in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.
Ações bioquímicas/fisiológicas
Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.
Características e benefícios
- Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
- High recovery rate with minimal sample dilution.
- Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
- Specifically designed as a BioProcess resin for industrial applications.
Informações legais
Sephadex is a registered trademark of Cytiva
substituído por
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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J J Knickerbocker et al.
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