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G75120

Sigma-Aldrich

Sephadex® G-75

Medium

Sinônimo(s):

Gel filtration media, Gel filtration resin

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About This Item

Número CAS:
37224-29-6
Número MDL:
MFCD00132772
Código UNSPSC:
23151817
NACRES:
NA.56

Nível de qualidade

200

técnica(s)

buffer exchange: suitable

Grupo ativo da matriz

phase

inchaço

1 g swells to 12-15 mL gel

diâmetro da esfera

40-120 μm

aplicação(ões)

life science and biopharma

compatibilidade

Cytiva

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Descrição geral

Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.

Aplicação

Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.

Características e benefícios

  • Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
  • Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
  • Features the highest exclusion limit of 80,000 within the Sephadex G-range.
  • Classic gel filtration resin.

Informações legais

Sephadex is a registered trademark of Cytiva

substituído por

Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Galle J, et al.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Oxidized low density lipoproteins potentiate vasoconstrictions to various agonists by direct interaction with vascular smooth muscle.
Galle J, et al.
Circulation Research, 66(5), 1287-1293 (1990)
Jose Munoz-Munoz et al.
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
K Krakowski et al.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
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Protein and peptide letters, 18(2), 210-218 (2010-11-09)
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. To purify and characterize Thiol protease inhibitor from buffalo brain and to compare its properties with respect to tissue and organ difference from other
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