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Documentos Principais

GE29-0005-93

His Spintrap TALON®

Cytiva 29-0005-93

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

fabricante/nome comercial

Cytiva 29-0005-93

embalagem

pkg of 50 x 100 μL

volume do leito

100 μL

Matriz

6% cross-linked agarose

tamanho de partícula

60-160 μm

cleaning

2-14(Co2+-stripped medium)

Faixa de trabalho

3-12(Co2+-stripped medium)

capacidade

≤1 mg binding capacity(histidine-tagged)
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

temperatura de armazenamento

2-8°C

Descrição geral

His SpinTrap Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Aplicação

His SpinTrap Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Características e benefícios

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Artigos

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protocolos

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

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