GE17-5282-01

GSTrap^™ High Performance

Cytiva 17-5282-01, pack of 1 × 5 mL

• Sinônimo(s): GSTrap Column
• Código UNSPSC: 23151816
• NACRES: NA.56

Propriedades

• ligand: glutathione
• embalagem: pack of 1 × 5 mL
• fabricante/nome comercial: Cytiva 17-5282-01
• Parâmetros: flow rate; <20 mL/min flow rate; 42 psi (H₂O at room temperature.)
• tamanho do leito: 16 mm × 25 mm
• volume do leito: 5 mL
• D.I. da coluna: 16 mm
• Matriz: highly cross-linked 6% agarose
• Tamanho médio da partícula: 34 μm
• cleaning: 3-12
• Faixa de trabalho: 3-12
• capacidade: >7 mg/mL binding capacity (medium)

Descrição

Descrição geral

GSTrap^™ HP columns are prepacked with Glutathione Sepharose^™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Aplicação

GSTrap^™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose^™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose^™ High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.

Características e benefícios

• High resolution purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Fast, one-step purification.
• Easy to use with syringe, pump or a chromatography system.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 1