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GE17-5248-01

HisTrap High Performance

Cytiva 17-5248-01, pack of 1 × 5 mL

Sinônimo(s):

High Performance HisTrap, HisTrap Column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

embalagem

pack of 1 × 5 mL

fabricante/nome comercial

Cytiva 17-5248-01

Parâmetros

4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacidade

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descrição geral

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

HisTrap HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.

The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.

Características e benefícios

  • High binding capacity, at least 40 mg/mL chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Environment

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Aquatic Chronic 3 - Carc. 2 - Flam. Liq. 3 - Skin Sens. 1 - STOT RE 1

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

100.4 - 109.4 °F - closed cup

Ponto de fulgor (°C)

38 - 43 °C - closed cup


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Principles and Standard Conditions for Different Purification Techniques

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

Protocolos

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

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