GE29-0510-21

HisTrap^™ High Performance

Cytiva 29-0510-21, pack of 1 mL

• Código UNSPSC: 23151816
• NACRES: NA.56

Propriedades

• embalagem: pack of 1 mL
• fabricante/nome comercial: Cytiva 29-0510-21
• disponibilidade: not available in North America
• Parâmetros: flow rate; 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature); 42 psi (H₂O at room temperature.)
• tamanho do leito: 7 mm × 25 mm
• volume do leito: 1 mL
• D.I. da coluna: 7 mm
• Matriz: highly cross-linked 6% agarose
• Tamanho médio da partícula: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• Faixa de trabalho: 3-12(Ni²⁺-stripped medium)
• capacidade: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.); ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descrição

Descrição geral

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Aplicação

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Características e benefícios

• High binding capacity, at least 40 mg/ml chromatography medium.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible Ni²⁺ leakage
• Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Informações legais

HisTrap is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS09
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids