GE28-9365-51

Hisprep^™ Fast Flow 16/10

Cytiva 28-9365-51

• Sinônimo(s): 16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column
• Código UNSPSC: 23151817
• NACRES: NA.56

Propriedades

• embalagem: pkg of 1 ea
• fabricante/nome comercial: Cytiva 28-9365-51
• Parâmetros: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• tamanho do leito: 16 mm × 100 mm
• volume do leito: 20 mL
• D.I. da coluna: 16 mm
• matriz: 6% cross-linked agarose
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacidade: ~40 mg binding capacity(histidine-tagged protein)
• adequação: suitable for bioprocess medium

Descrição

Descrição geral

HisPrep^™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep^™ FF 16/10 columns are prepacked with Ni Sepharose^™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose^™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Características e benefícios

• Optimized for convenient scale-up purification of histidine-tagged proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Negligible leakage of Ni²⁺.
• High binding capacity, approx. 40 mg/mL medium.
• Convenient and time-saving 20 mL prepacked HiPrep^™ format.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS09
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids