GE28-9782-44

HiScreen^™ Ni Fast Flow

Cytiva 28-9782-44

• Código UNSPSC: 13111000
• NACRES: NA.56

Propriedades

• embalagem: pkg of 1 ea
• fabricante/nome comercial: Cytiva 28-9782-44
• Parâmetros: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• tamanho do leito: 7.7 mm × 100 mm
• volume do leito: 4.7 mL
• D.I. da coluna: 7.7 mm
• Matriz: 6% cross-linked agarose
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• Faixa de trabalho: 3-12(Ni²⁺-stripped medium.)
• capacidade: ~40 mg binding capacity(histidine-tagged protein)
• adequação: suitable for bioprocess medium

Descrição

Descrição geral

HiScreen^™ columns prepacked with Ni Sepharose^™ 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Aplicação

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen^™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose^™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

Características e benefícios

• High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
• Prepacked, ready-to-use columns for convenient process development.
• Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
• Easy connection in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS09
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids