GE28-9365-49

HiPrep^™ Heparin Fast Flow 16/10

Cytiva 28-9365-49

• Sinônimo(s): Heparin affinity chromatography
• Código UNSPSC: 13111000
• NACRES: NA.56

Propriedades

• ligand: porcine heparin
• Formulário: prepacked column
• reticulação: cross-linked
• embalagem: pkg of 1 ea
• fabricante/nome comercial: Cytiva 28-9365-49
• condição de armazenamento: (20% ethanol containing 0.05 M sodium acetate)
• Parâmetros: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 2 ml/min - 10 ml/min flow rate; 22 psi
• técnica(s): liquid chromatography (LC): suitable
• tamanho do leito: 16 mm × 100 mm
• volume do leito: 20 mL
• D.I. da coluna: 16 mm
• Matriz: 6% cross-linked agarose
• Grupo ativo da matriz: heparin
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 4-13
• Faixa de trabalho: 4-12
• adequação: suitable for bioprocess medium
• técnica de separação: affinity

Descrição

Descrição geral

HiPrep^™ Heparin FF 16/10 is prepacked with Heparin Sepharose^™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Aplicação

The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose^™ 6 Fast FLow with a chemically optimized linkage.

Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.

Características e benefícios

• 20 mL column optimized for convenient scale-up purification .
• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins.
• Simple operation with single pump configurations, as well as ÄKTA^® design systems.
• Excellent flow characteristics.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)

Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiPrep is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids