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GE17-5318-06

Ni Sepharose 6 Fast Flow

Cytiva 17-5318-06, pack of 5 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

embalagem

pack of 5 mL

fabricante/nome comercial

Cytiva 17-5318-06

Matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

Faixa de trabalho

3-12(Ni2+-stripped medium.)

capacidade

~40 mg binding capacity(histidine-tagged protein)

adequação

suitable for bioprocess medium

Descrição geral

Ni Sepharose 6 Fast FLow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast FLow platform.

Aplicação

Ni Sepharose 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged Proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.

Ni Sepharose 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • Designed for scaling up purification of histidine-tagged Proteins
  • High Protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Environment

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Artigos

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Protocolos

This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.

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