GE17-5318-06
Ni Sepharose™ 6 Fast Flow
Cytiva 17-5318-06, pack of 5 mL
About This Item
Produtos recomendados
embalagem
pack of 5 mL
fabricante/nome comercial
Cytiva 17-5318-06
Matriz
6% cross-linked agarose
tamanho de partícula
45-165 μm
diâmetro médio
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
Faixa de trabalho
3-12(Ni2+-stripped medium.)
capacidade
~40 mg binding capacity(histidine-tagged protein)
adequação
suitable for bioprocess medium
Categorias relacionadas
Descrição geral
Aplicação
Ni Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged Proteins.
The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.
Ni Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Características e benefícios
- Designed for scaling up purification of histidine-tagged Proteins
- High Protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
Armazenamento e estabilidade
Nota de análise
Informações legais
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
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Artigos
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
Protocolos
This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.
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