GE27-0843-01
PreScission Protease
Cytiva 27-0843-01
Sinônimo(s):
fusion protein, GST-tag removal
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About This Item
Código UNSPSC:
41106501
NACRES:
NA.56
Produtos recomendados
embalagem
pkg of 500 units
fabricante/nome comercial
Cytiva 27-0843-01
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Categorias relacionadas
Descrição geral
PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step.
On-column or off-column enzyme cleavage
GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.
Vectors encoding the HRV 3C protease recognition sequence
pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.
On-column or off-column enzyme cleavage
GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.
Vectors encoding the HRV 3C protease recognition sequence
pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.
Aplicação
Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.
Características e benefícios
- Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
- Time-saving – GST-tagged proteins can be cleaved while still bound to an affinity resin.
- Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence.
Armazenamento e estabilidade
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Nota de análise
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Informações legais
pGEX Vectors
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Certificados de análise (COA)
Lot/Batch Number
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Artigos
This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.
Protocolos
Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)
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