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Key Documents

GE27-0846-01

Thrombin Protease

Cytiva 27-0846-01

Sinônimo(s):

Proteolytic Enzyme, Thrombin Activity, Thrombin Enzyme

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.56

embalagem

pkg of 500 units

fabricante/nome comercial

Cytiva 27-0846-01

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma.

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors (see pGEX Vectors (GST Gene Fusion System)) containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Aplicação

Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Características e benefícios

  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose™ High Performance, or Glutathione Sepharose™ 4 Fast Flow

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palavra indicadora

Danger

Frases de perigo

H315,H319,H334,H335

Classificações de perigo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Thrombin, Bovine

Sigma-Aldrich

605157

Thrombin, Bovine

Trombina lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

Sigma-Aldrich

T6884

Trombina

Protease de TEV

Sigma-Aldrich

T4455

Protease de TEV

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Artigos

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

Ver tudo

Protocolos

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

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