71493-M

HRV 3C Protease

Highly efficient fusion tag removal at low temperature

• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• Nível de qualidade: 400
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• concentração: 1.8-3.0 unit/μL
• Condições de expedição: dry ice; wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Recombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln?GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His Tag^® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations withHis Tag^®, S•Tag, thioredoxin (Trx•Tag), glutathione-S-transferase (GST•Tag), and NusA (Nus•Tag) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect pIEx-9 vector, and the multisystem pTriEx-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Aplicação

• One unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.

Componentes

• 500 U - HRV 3C Protease
• 10 µg - HRV 3C Cleavage Control Protein
• 10 ml - 10X HRV 3C Cleavage Buffer

Advertência

Toxicity: Standard Handling

forma física

In 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

Informações legais

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable