G8160
Granulocyte Colony-Stimulating Factor from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinônimo(s):
G-CSF
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About This Item
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Nome do produto
Granulocyte Colony-Stimulating Factor from mouse, G-CSF, recombinant, expressed in E. coli, suitable for cell culture
fonte biológica
mouse
Nível de qualidade
recombinante
expressed in E. coli
Ensaio
>97% (SDS-PAGE)
Formulário
lyophilized powder
potência
10-60 pg/mL ED50
qualidade
endotoxin tested
peso molecular
19 kDa (179 amino acids including N-terminal methionine)
embalagem
pkg of 5 and 25 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
técnica(s)
cell culture | mammalian: suitable
Impurezas
≤0.01 EU/μg
nº de adesão UniProt
temperatura de armazenamento
−20°C
Informações sobre genes
mouse ... Csf3(12985) , Csf3r(12986)
Descrição geral
The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.
Aplicação
Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.
Ações bioquímicas/fisiológicas
G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.5 Human and murine G-CSF have about 75% homology and show biological cross-reactivity.
forma física
Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.
Nota de análise
The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
Informações legais
TWEEN is a registered trademark of Croda International PLC
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Descrição
Preços
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Hitesh S Deshmukh et al.
Nature medicine, 20(5), 524-530 (2014-04-22)
Neonatal colonization by microbes, which begins immediately after birth, is influenced by gestational age and the mother's microbiota and is modified by exposure to antibiotics. In neonates, prolonged duration of antibiotic therapy is associated with increased risk of late-onset sepsis
Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice
Christopher MJ, et al.
The Journal of Experimental Medicine (2011)
Combining pharmacological mobilization with intramyocardial delivery of bone marrow cells over-expressing VEGF is more effective for cardiac repair
Wang Y, et al.
Journal of Molecular and Cellular Cardiology, 40(5), 736-745 (2006)
Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice
ChristopherMJ, et al.
The Journal of Experimental Medicine (2011)
Rui Zhang et al.
Hematology (Amsterdam, Netherlands), 20(10), 555-560 (2015-06-13)
The treatment for acute myeloid leukemia (AML) remains an important clinical problem. Recently, hematopoietic stem cell therapy provides promising outcomes. In this study we examined the clinical effect of HLA-mismatched hematopoietic stem cell infusion combined with chemotherapy as a postremission
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