H5666
Granulocyte-Macrophage Colony-Stimulating Factor human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Sinônimo(s):
GM-CSF human
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About This Item
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Nome do produto
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
fonte biológica
human
Nível de qualidade
recombinante
expressed in HEK 293 cells
Ensaio
≥95% (SDS-PAGE)
Formulário
lyophilized powder
potência
≤5 ng/mL EC50
qualidade
endotoxin tested
peso molecular
dimer 15-36 kDa (glycosylated)
embalagem
pkg of 5X10 μg
pkg of 10 μg
condição de armazenamento
avoid repeated freeze/thaw cycles
técnica(s)
cell culture | mammalian: suitable
Impurezas
≤1 EU/mg
nº de adesão UniProt
temperatura de armazenamento
−20°C
Informações sobre genes
human ... CSF2(1437)
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Descrição geral
Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.
Aplicação
Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).
Ações bioquímicas/fisiológicas
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Atenção
Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
forma física
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Nota de preparo
HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Nota de análise
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Informações legais
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Artigos
Read article on hematopoietic cytokines and hematopoiesis
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