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G0407

Sigma-Aldrich

Granulocyte colony-stimulating factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

product name

Granulocyte colony-stimulating factor human, G-CSF, recombinant, expressed in E. coli, suitable for cell culture

fonte biológica

human

Nível de qualidade

recombinante

expressed in E. coli

Ensaio

≥95% (SDS-PAGE)

forma

lyophilized powder

potência

0.01-0.1 ng/mL

qualidade

endotoxin tested

peso molecular

18.8 kDa (175 amino acids including N-terminal methionine)

embalagem

pkg of 5 and 25 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<1 EU/μg (LAL test)

nº de adesão UniProt

temperatura de armazenamento

−20°C

Informações sobre genes

human ... CSF3(1440)

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Descrição geral

Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.

Aplicação

Granulocyte colony-stimulating factor human has been used:
  • in culturing human neutrophils
  • as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells
  • to test its protective effects chronic liver damage model

Ações bioquímicas/fisiológicas

Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).
Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

forma física

Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF

Nota de análise

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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