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SRP3044

Sigma-Aldrich

Flt-3 Ligand human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinônimo(s):

Fms-related tyrosine kinase 3 ligand

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥98% (HPLC)
≥98% (SDS-PAGE)

Formulário

lyophilized

potência

≤10 ng/mL ED50 ((OCI-AML5 cell proliferation))

peso molecular

17.6 kDa

embalagem

pkg of 10 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

white to off-white

Descrição geral

FLT3LG (Fms-related tyrosine kinase 3 ligand) is a transmembrane glycoprotein and a ligand for FLT3 receptor.[1] It is widely expressed in human tissues. The protein exists in membrane bound as well as secreted forms.[2] Binding of FLT3LG to FLT3 results in receptor dimerization, activation of the tyrosine kinase domain, autophosphorylation and thereby activation of the receptor.[3] Recombinant human Flt3-Ligand is a soluble 17.6kDa protein consisting of 155 amino acid residues.

Ações bioquímicas/fisiológicas

FLT3LG (Fms-related tyrosine kinase 3 ligand) is a hematopoietic growth factor. It plays a significant role in the development of myeloid and plasmacytoid dendritic cells. It is also involved in the expansion of Treg (T regulatory) cells, thereby offering treatment for autoimmune disorders and transplantation processes.[4] FLT3LG is associated with autoimmune arthritis, for instance rheumatoid arthritis and primary Sjogren′s syndrome.[1] FLT3LG along with FLT3 is involved in the proliferation of primary AML (acute myeloid leukemia) blasts and autocrine signaling in AML.[3]

Sequência

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT

forma física

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Os clientes também visualizaram

Further activation of FLT3 mutants by FLT3 ligand.
Zheng R, et al.
Oncogene, 30, 4004-4004 (2011)
FMS-related tyrosine kinase 3 ligand (Flt3L)/CD135 axis in rheumatoid arthritis.
Ramos MI, et al.
Arthritis Research & Therapy, 15, R209-R209 (2013)
Flt3 ligand expands CD4+ FoxP3+ regulatory T cells in human subjects.
Klein O, et al.
European Journal of Immunology, 43, 533-533 (2013)
Serum concentrations of Flt-3 ligand in rheumatic diseases.
Nakamura K, et al.
Bioscience Trends, 9, 342-342 (2015)
K Mackarehtschian et al.
Immunity, 3(1), 147-161 (1995-07-01)
The flk2 receptor tyrosine kinase has been implicated in hematopoietic development. Mice deficient in flk2 were generated. Mutants developed into healthy adults with normal mature hematopoietic populations. However, they possessed specific deficiencies in primitive B lymphoid progenitors. Bone marrow transplantation

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