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H5416

Sigma-Aldrich

FLT3 Ligand human

HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

Nome do produto

FLT3 Ligand human, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

fonte biológica

human

Nível de qualidade

recombinante

expressed in HEK 293 cells

Ensaio

≥95% (SDS-PAGE)

Formulário

lyophilized powder

potência

≤0.8 ng/mL EC50

qualidade

endotoxin tested

peso molecular

monomer 24-30 kDa (glycosylated)

embalagem

pkg of 10 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤1 EU/mg

temperatura de armazenamento

−20°C

Descrição geral

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Ações bioquímicas/fisiológicas

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.
HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

forma física

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Nota de análise

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Informações legais

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Visite a Biblioteca de Documentos

Chunaram Choudhary et al.
International journal of hematology, 82(2), 93-99 (2005-09-09)
Activating mutations of Fms-like tyrosine kinase 3 (Flt3) are the most common genetic lesions in acute myeloid leukemia (AML) and are present in approximately one third of AML patients. The 2 classes of Flt3 mutations are internal tandem duplications in
Nobuyuki Onai et al.
Annals of the New York Academy of Sciences, 1106, 253-261 (2007-03-16)
Flt3-ligand is a nonredundant cytokine in type I interferon-producing cell (IPC) and dendritic cell (DC) development. We demonstrated that IPC and DC differentiation potential is confined to Flt3(+)-hematopoietic progenitor cells, that Flt3-ligand drives development along both lymphoid and myeloid developmental
Structure-function analysis of FLT3 ligand-FLT3 receptor interactions using a rapid functional screen
Graddis T, et al.
The Journal of Biological Chemistry, 273(28), 17626-17633 (1998)
Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots
Savvides S N, et al.
Nature Structural and Molecular Biology, 7(6), 486-486 (2000)

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