SRP3151

SCF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinônimo(s): Steel Factor, Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF)
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• forma: lyophilized
• potência: ≤2.0 ng/mL ED₅₀
• peso molecular: 18.4 kDa
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white
• nº de adesão UniProt: P21583
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... KITLG(4254)

Descrição

Descrição geral

SCF (stem cell factor) is a hematopoietic cytokine that exists in both soluble and transmembrane forms, which arise due to alternative splicing. Both these forms are functionally active. This gene is localized to human chromosome 12q22-12q24. Endothelial cells and fibroblasts constitutively produce both transmembrane and soluble forms of SCF. It is also produced by keratinocytes in normal skin, and epithelial cells in the gut, and thymus. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Ações bioquímicas/fisiológicas

SCF (stem cell factor) exerts its activity by signaling through the c-Kit receptor. They participate in hematopoiesis, and development of mast cells and melanocytes. Mutations in either of these proteins result in various phenotypic disorders, such as varying degrees of macrocytic anemia, suppressed numbers of tissue mast cells, reduced fertility, and decreased pigmentation. Linkage analysis along with whole-exome sequencing, c.286_303delinsT (p.Ser96Ter), shows a heterozygous truncating mutation in this gene linked with congenital and stable non-syndromic unilateral and asymmetric hearing loss. rs4590952 polymorphism in this gene is linked with disruption of p53 function through increase in p53-dependent SCF expression, which results in increased susceptibility to testicular cancer.

Sequência

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

forma física

Lyophilized from 10 mM Acetic Acid.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319
• Declarações de precaução: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable