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D0547

Sigma-Aldrich

Meio de Eagle modificado por Dulbecco/Mistura de nutrientes de Ham F-12

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

Mistura 1:1 de DME/F-12

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.71

Nível de qualidade

Formulário

powder

técnica(s)

cell culture | mammalian: suitable

componentes

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Aplicação

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Outras notas

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantidade

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstituição

Supplement with 1.2 g/L sodium bicarbonate

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