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D2906

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Sinônimo(s):

Mistura 1:1 de DME/F-12

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

product name

Meio de Eagle modificado por Dulbecco/Mistura de nutrientes de Ham F-12, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Nível de qualidade

forma

powder

técnica(s)

cell culture | mammalian: suitable

componentes

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Aplicação

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Quantidade

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstituição

Supplement with 1.2 g/L sodium bicarbonate.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


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