A6132
Apirase
ATPase ≥3.0 units/mg protein, lyophilized powder
Sinônimo(s):
Adenosina 5′ difosfatase, Adenosina 5′ trifosfatase
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About This Item
Produtos recomendados
fonte biológica
potato
Nível de qualidade
tipo
Grade I
Formulário
lyophilized powder
ATPase activity
≥3.0 units/mg protein
composição
Protein, 30-60%
temperatura de armazenamento
−20°C
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Aplicação
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Pelo menos duas isoenzimas são encontradas em diferentes variedades de S. tuberosum: uma com alta proporção de ATPase/ADPase (∼10) e outra com uma baixa proporção (∼1).
Reação: ATP → ADP+Pi → AMP+2Pi.
Reação: ATP → ADP+Pi → AMP+2Pi.
Ações bioquímicas/fisiológicas
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Embalagem
Sold on the basis of ATPase units.
Outras notas
Mixture of high and low ratio isoenzymes.
Definição da unidade
One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C
forma física
Partially purified, lyophilized powder containing potassium succinate
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Nucleoside triphosphate diphosphohydrolases (NTPDases; apyrases) (EC 3.6.1.5) hydrolyze di- and triphosphate nucleotides, but not monophosphate nucleotides. They are categorized as E-type ATPases, have a broad divalent cation (Mg(2+), Ca(2+)) requirement for activation and are insensitive to inhibitors of F-type, P-type
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