A6410
Apirase
ATPase ≥200 units/mg protein, lyophilized powder
Sinônimo(s):
Adenosina 5′ difosfatase, Adenosina 5′ trifosfatase
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About This Item
Produtos recomendados
tipo
Grade VI
Nível de qualidade
forma
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
peso molecular
~49 kDa by gel filtration
composição
Protein, 30-60%
solubilidade
H2O: soluble 1.0 mg/mL
atividade externa
Acid Phosphatase ≤2% of base activity
temperatura de armazenamento
−20°C
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Descrição geral
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Aplicação
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Pelo menos duas isoenzimas são encontradas em diferentes variedades de S. tuberosum: uma com alta proporção de ATPase/ADPase (∼10) e outra com uma baixa proporção (∼1).
Reação: ATP → ADP+Pi → AMP+2Pi.
Reação: ATP → ADP+Pi → AMP+2Pi.
Ações bioquímicas/fisiológicas
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Embalagem
Sold on the basis of ATPase units.
Outras notas
This grade has an exceptionally high ATPase/ADPase ratio.
Definição da unidade
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
forma física
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Nota de preparo
Derived from white potato
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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