A7646
Apirase
ATPase ≥60 units/mg protein, lyophilized powder (partially purified)
Sinônimo(s):
Adenosina 5′ difosfatase, Adenosina 5′ trifosfatase
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About This Item
Produtos recomendados
fonte biológica
potato
Nível de qualidade
tipo
Grade III
Formulário
lyophilized powder (partially purified)
ATPase activity
≥60 units/mg protein
peso molecular
~49 kDa by gel filtration
composição
Protein, 30-60%
solubilidade
H2O: soluble 10 mg/mL, slightly hazy
aplicação(ões)
diagnostic assay manufacturing
atividade externa
Acid Phosphatase ≤5% of base activity
temperatura de armazenamento
−20°C
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Aplicação
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
Pelo menos duas isoenzimas são encontradas em diferentes variedades de S. tuberosum: uma com alta proporção de ATPase/ADPase (∼10) e outra com uma baixa proporção (∼1).
Reação: ATP → ADP+Pi → AMP+2Pi.
Reação: ATP → ADP+Pi → AMP+2Pi.
Ações bioquímicas/fisiológicas
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.
Embalagem
Sold on the basis of ATPase units
Outras notas
Mixture of high and low ratio isoenzymes.
Qualidade
Contains ≤ 5% acid phosphatase.
Definição da unidade
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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