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Key Documents

F1904

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, purified 120 kDa α Chymotryptic Fragment (cell attachment region), suitable for cell culture

Sinônimo(s):

Fibronectin Fragment, Human Fibronectin

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified,

fonte biológica

human

Nível de qualidade

forma

lyophilized

peso molecular

120 kDa

fabricante/nome comercial

Chemicon®

concentração

1 mg/mL

técnica(s)

cell culture | mammalian: suitable

entrada

sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... FN1(2335)

Descrição geral

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.
Product Source: Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Aplicação

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

forma física

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

Armazenamento e estabilidade

Maintain material at -20°C for 3 months or -70°C for six months.

Nota de análise

One major band at 120kDa in SDS-PAGE

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1


Certificados de análise (COA)

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Os clientes também visualizaram

Slide 1 of 3

1 of 3

Fibronectin: purification, immunochemical properties, and biological activities.
Ruoslahti, E, et al.
Test, 82 Pt A, 803-831 (1982)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

Artigos

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Conteúdo relacionado

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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