L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• Código UNSPSC: 12352200
• NACRES: NA.21

Propriedades

• tipo: for DNA synthesis
• Nível de qualidade: 200
• linha de produto: Proligo Reagents
• Formulário: liquid
• condição de armazenamento: dry at room temperature
• Impurezas: ≤100 ppm water content (Karl Fischer)
• cor: clear
• adequação: conforms to structure (GC, Formulation)
• compatibilidade: configured for (ÄKTA^® and OligoPilot^®)
• temperatura de armazenamento: room temp

Descrição

Aplicação

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Nota de preparo

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Outras notas

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

Informações legais

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02,GHS06,GHS08,GHS05
• Palavra indicadora: Danger
• Frases de perigo: H225,H302 + H332,H311,H314,H361d
• Declarações de precaução: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): 35.6 °F
• Ponto de fulgor (°C): 2 °C