T7924

Transforming Growth Factor-α human

TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): hTGF-α, TGF-α
• Empirical Formula (Hill Notation): C₂₃₉H₃₄₈N₇₀O₇₂S₆
• CAS Number: 105186-99-0
• Molecular Weight: 5546.14
• MDL number: MFCD00082077
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE and HPLC)
• form: powder
• potency: ≤0.2 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 5.5 kDa
• packaging: pkg of 1 mg; pkg of 5 mg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P01135
• storage temp.: −20°C
• Gene Information: human ... TGFA(7039)

Description

Amino Acid Sequence

Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]

General description

The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33. TNFα is a member of TNF superfamily.

Application

Transforming Growth Factor-α human has been used:

• to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles
• in human hepatocytes cell culture and keratinocyte cell culture
• to induce morphogenesis in primary mouse organoids

Biochem/physiol Actions

The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.

Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorpation into BALB/c 3T3 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)