I3769

Insulin-like Growth Factor-I human

IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IGF, IGF-I, somatomedin c
• MDL number: MFCD00081716
• NACRES: NA.77

Properties

• Quality Level: 200
• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC)
• form: lyophilized powder
• potency: 0.1-10 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: ~7.6 kDa
• packaging: pkg of 50 and 100 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.00 EU/μg
• UniProt accession no.: P05019
• storage temp.: −20°C
• Gene Information: human ... IGF1(3479) , Igf1(16000)

Description

General description

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.

Application

Insulin-like Growth Factor-I human has been used:

• in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
• in culture media, to study its effect on steroidogenesis during testicular development
• in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.

Physical form

Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.

Reconstitution

Reconstitute in water

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED₅₀ corresponds to a specific activity of at least 1 x 10⁵ units/mg.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves