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F4021

Sigma-Aldrich

Follicle Stimulating Hormone from human pituitary

~7,000 IU/mg (vs. W.H.O. reference preparation 83/575)

Synonym(s):
FSH
CAS Number:
MDL number:
eCl@ss:
42020504
NACRES:
NA.32

sterility

non-sterile

Quality Level

form

powder

specific activity

~7,000 IU/mg (vs. W.H.O. reference preparation 83/575)

mol wt

35 kDa

impurities

HBsAg, HCV and HIV-1/HIV-2, tested negative

solubility

water: ~50 g/L

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

General description

FSH (follicle stimulating hormone) protein is composed of two subunits- α and β, where a subunit has resemblance to LH (luteinizing hormone), hCG (human chorionic gonadotropin) and TSH (thyrotropin-releasing hormone), which are glycoprotein hormones. α subunit has one glycosylation site and β subunit has two.
The α chain of FSH has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.[49]

Application

Follicle stimulating hormone (FSH) from human pituitary has been used for-
  • in vitro luteinization of Macaque granulosa cells
[06]
  • luteal cell proliferation assay
  • [52]
  • culturing of preantral follicle cells obtained from mice
  • [08]
  • in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
  • [23]
  • culture of RAW264.7 cell line
  • [62]

    Biochem/physiol Actions

    FSH (follicle stimulating hormone) in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle stimulating hormone receptor) interacts with FSH in the ovaries, and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis.[99] In Caucasian population, certain polymorphisms in FSHR gene are more commonly found in patients with polycystic ovary syndrome (PCOS).[98] In male fetus and neonates, it facilitates the proliferation of Sertoli cells. In pubescent males, it stimulates spermatogonia to undergo mitosis.[05]
    The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

    Packaging

    Package size based on protein content

    Other Notes

    Two-chain glycoprotein hormone

    Quality

    Vial also contains 0.1 mg of salts from 0.05 M phosphate buffer, pH 7.4.

    Storage Class Code

    13 - Non Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificate of Analysis

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    Certificate of Origin

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    Product Information Sheet

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