H8916
Tumor Necrosis Factor-α human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
TNF-α
About This Item
Recommended Products
product name
Tumor Necrosis Factor-α human, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤1.0 ng/mL ED50
quality
endotoxin tested
mol wt
17 kDa (glycosylated)
~17.4 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg (endotoxin)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TNF(7124)
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General description
Application
- In induction of netting neutrophils by anti-neutrophil cytoplasmic antibody and to study its effect on platelet activation and formation of monomeric C-reactive protein.
- To study the effect of TNF-α on miR-221 and fractalkine expression.
- To induce inflammatory cell responses.
- In NF-κB luciferase reporter assay.
- as a permeability inducing agent for endothelial cell monolayer permeability assay
- as a reactive oxygen species inducer in primary rat cardiac microvascular endothelial cells (RCMVECs)
- in the activation of nuclear factor kappa B (NF-κB) in human embryonic kidney cells (HEK293), neuroblastoma SH-SY5Y cells and HeLa cells
- in the stimulation of the human keratinocyte cell line(HaCaT) and human coronary artery endothelial cells (HCAECs)
Biochem/physiol Actions
Preparation Note
Analysis Note
comparable product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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