807477
TOYOPEARL® Butyl-650M Bulk Media
matrix polymer (65 μm), bottle of 100 mL, 65 μm
Synonim(y):
TOYOPEARL® Butyl-650 HIC Bulk Media
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
41115700
Polecane produkty
opis
Butyl-650M
linia produktu
TOYOPEARL®
Formularz
slurry
opakowanie
bottle of 100 mL
Parametry
3 bar max. pressure
metody
HPLC: suitable
Matryca
polymer (65 μm)
grupa aktywna macierzy
C4 phase
wielkość cząstki
65 μm
pH robocze
1-13
pojemność
40 g/L±10 adsorption capacity (lysozyme)
metoda separacji
hydrophobic interaction (HIC)
Opis ogólny
TOYOPEARL® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.
TOYOPEARL® HIC resins for hydrophobic interaction chromatography offer these advantages:
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.
- Strong affinity for water-soluble proteins
- High recovery of mass and activity
- High sample capacity: up to 2-4 times that of gel media
- Fluctuating salt concentrations will not change bed volume
- Mechanical stability to 7kg/cm2 (7 bar/100psi)
- Stable at wide pH range: 2-12
- Clean in place with 0.5M NaOH
- Autoclavable
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.
Zastosowanie
Nośniki TOYOPEARL® są stosowane w nośnikach do oddziaływań hydrofobowych, żywicach i nośnikach separacyjnych. Nośniki TOYOPEARL® oferują wysoką wydajność odzyskiwania białek przy użyciu różnych eluentów wodnych.
Charakterystyka techniczna
Oczyścić na miejscu za pomocą 0,5 M NaOH lub 0,1 M HCl.
Postać fizyczna
Wysyłany w 20% (v/v) etanolu.
Inne uwagi
Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps
Informacje prawne
Toyopearl is a registered trademark of Tosoh Corporation
Ta strona może zawierać tekst przetłumaczony maszynowo.
produkt powiązany
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Flam. Liq. 3
Klasa zagrożenia wodnego (WGK)
WGK 1
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.
Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Hongwei Luan et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 24(5), 867-873 (2008-08-30)
A beta-D-xylosidase from Leifsonia shinshuensis DICP 16 was purified to apparent homogeneity using a combination of ammonium sulfate precipitation, DE 52 anion-exchange, Q-Sepharose Fast Flow anion-exchange, Toyopearl Butyl 650C hydrophobic-interaction and Sephacryl S-300 HR gel-permeation chromatography. The purified xylosidase consisted
Hydrophobic interaction chromatography selectivity changes among three stable proteins: conformation does not play a major role
Jones, T.T. and Fernandez, E.J.
Biotechnology and Bioengineering, 87, 288-299 (2004)
V Stocchi et al.
Journal of chromatography. A, 676(1), 51-63 (1994-07-29)
Hydrophobic interaction chromatography (HIC) has been employed extensively in the separation of proteins by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this study, a new hydrophobic interaction chromatographic support, Toyopearl
P Ceccaroli et al.
Journal of chromatography. B, Biomedical sciences and applications, 702(1-2), 41-48 (1998-02-04)
Hydrophobic interaction chromatography (HIC) has been used extensively for the separation of proteins and peptides by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this paper we compare different hydrophobic interaction
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej