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RIP

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Imprint® RNA Immunoprecipitation Kit

High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

Synonim(y):

Immunoprecypitacja RNA, Immunoprecypitacja RNA za pomocą kulek magnetycznych, Immunoprecypitacja mRNA, Immunoprecypitacja mikroRNA

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About This Item

Kod UNSPSC:
41116158
NACRES:
NA.54

Poziom jakości

Powiązane kategorie

Opis ogólny

Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.

Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.

Zastosowanie

Imprint® RNA Immunoprecipitation Kit has been used for:
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Cechy i korzyści

  • Xtra Protein A magnetic beads with higher binding capacity
  • Step-by-step protocols for cell lysis and immunoprecipitation
  • RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
  • Negative control and antibody included

Informacje prawne

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderKarta charakterystyki

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderKarta charakterystyki

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionKarta charakterystyki

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionKarta charakterystyki

  • R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLKarta charakterystyki

Piktogramy

FlameExclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Kod klasy składowania

3 - Flammable liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

100.4 °F

Temperatura zapłonu (°C)

38 °C


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Zhi-Jun Zhu et al.
Biochemical and biophysical research communications, 497(4), 971-977 (2018-02-11)
Long non-coding RNAs are critically involved in oncogenesis in various cancer types. Here we reported a novel lncRNA signature correlated with progression of non-small cell lung cancer (NSCLC). In particular, we identified elevated expression of terminal differentiation-induced noncoding RNA (TINCR)
Huijuan Xiao et al.
Cell & bioscience, 8, 23-23 (2018-03-29)
The acquisition of drug resistance has been considered as a main obstacle for cancer chemotherapy. Tumor protein 53 target gene 1 (TP53TG1), a p53-induced lncRNA, plays a vital role in the progression of human cancers. However, little is known about
Yu-Yuan Qian et al.
Oncotarget, 8(64), 107859-107869 (2018-01-04)
Long non-coding RNA PTENP1, the pseudogene of PTEN tumor suppressor, was previously reported to be a tumour suppressor in some cancer types. However, the precise effects mediated by PTENP1 transcripts within intricate regulatory networks involving molecular interactions with PTEN and
Nicola Amodio et al.
Leukemia (2018-03-01)
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance and the druggability of the oncogenic lncRNA MALAT1 in MM. Targeting MALAT1 by novel
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The

Produkty

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Protokoły

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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