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R1158
Ribonuclease Inhibitor
recombinant, expressed in E. coli, 20-40 units/μL
Synonim(y):
RNAse inhibitor
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About This Item
Polecane produkty
rekombinowane
expressed in E. coli
Poziom jakości
klasa czystości
for molecular biology
Formularz
buffered aqueous solution
masa cząsteczkowa
~50 kDa
stężenie
20-40 units/μL
Warunki transportu
dry ice
temp. przechowywania
−20°C
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Opis ogólny
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).
Zastosowanie
Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:
- cDNA synthesis from mRNA
- reverse transcriptase polymerase chain reaction (RT-PCR)
- in vitro transcription and translation
- mRNA protection from degradation
- formaldehyde cross-linked lncRNA purification
- RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Działania biochem./fizjol.
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Komponenty
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Definicja jednostki
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Postać fizyczna
Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.
Inne uwagi
- Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
- This product is for R&D use only, not for drug, household, or other uses.
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produkt powiązany
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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