R2520
Ribonuclease inhibitor human
Inhibits ribonucleases during transcription and translation experiments
Synonim(y):
Inhibitor RNazy, Inhibitor rybonukleazy
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About This Item
Polecane produkty
pochodzenie biologiczne
human placenta
klasa czystości
for molecular biology
Formularz
buffered aqueous solution
masa cząsteczkowa
~50 kDa
stężenie
30,000-50,000 units/mL
Warunki transportu
dry ice
temp. przechowywania
−20°C
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Opis ogólny
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is human placenta. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes.
Zastosowanie
Suitable for use in
- in vitro inhibition of ribonucleases
- cDNA synthesis
- RT-PCR
- in vitro transcription and translation
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Działania biochem./fizjol.
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Inhibits RNase by forming a tight, non-covalent 1:1 complex. Also inhibits angiogenin, a neovascularization promoter which is homologous with RNase A.
Komponenty
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Inne uwagi
This inhibitor of RNase activity is isolated from human placenta and supplied as a buffered aqueous glycerol solution.
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
Definicja jednostki
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Postać fizyczna
Solution in 50% glycerol, 20 mM HEPES-KOH, pH 7.6, 50 mM KCl and 8 mM DTT
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Numer produktu
Opis
Cennik
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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