RNASEA-RO
Roche
RNase A
from bovine pancreas
Synonim(y):
Rnase
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About This Item
Polecane produkty
pochodzenie biologiczne
bovine pancreas
Poziom jakości
Postać
dry powder
aktywność właściwa
50 U/mg (+25°C, RNA as substrate)
masa cząsteczkowa
Mr = 13,700
opakowanie
pkg of 100 mg (10109169001)
pkg of 25 mg (10109142001)
producent / nazwa handlowa
Roche
Parametry
65 °C optimum reaction temp.
metody
flow cytometry: suitable
optymalne pH
7.0-7.5
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.
Zastosowanie
- For analytical purposes
- Isolation of DNA (for this purpose, RNase A should be boiled)
- For cell cycle analysis by flow cytometry and propidium iodide (PI) staining
Działania biochem./fizjol.
RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.
Definicja jednostki
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Rekonstytucja
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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