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P2308

Sigma-Aldrich

Proteinase K from Tritirachium album

lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

Synonim(y):

Endopeptidase K

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About This Item

Numer CAS:
39450-01-6
Numer EC enzymu:
3.4.21.64 (BRENDA, IUBMB)
Numer WE:
254-457-8
Numer MDL:
MFCD00132129
Kod UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

klasa czystości

for molecular biology

Poziom jakości

400

linia produktu

BioUltra

Postać

lyophilized powder

aktywność właściwa

≥30 units/mg protein

masa cząsteczkowa

28.93 kDa

skład

Protein, ≥90% biuret

zanieczyszczenia

≤1 ppm DNA

obecność zanieczyszczeń

DNAse, Nickase and RNAse, none detected

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Zastosowanie

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Działania biochem./fizjol.

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Definicja jednostki

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
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Piktogramy

Exclamation markHealth hazard
GHS07,GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H315 - H319 - H334 - H335

Zwroty wskazujące środki ostrożności

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certyfikaty analizy (CoA)

Lot/Batch Number
SLBX1525
089M4033V
50180408
SLCH8204
SLCH8209

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Mamoru Oshiki et al.
Frontiers in microbiology, 9, 830-830 (2018-05-15)
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed
Ruobing Jia et al.
Molecular cancer, 18(1), 161-161 (2019-11-15)
Dynamic N6-methyladenosine (m6A) RNA modification generated and erased by N6-methyltransferases and demethylases regulates gene expression, alternative splicing and cell fate. Ocular melanoma, comprising uveal melanoma (UM) and conjunctival melanoma (CM), is the most common primary eye tumor in adults and
Margarita Zayas et al.
PLoS pathogens, 12(1), e1005376-e1005376 (2016-01-05)
Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII
Bahram Peivastegan et al.
BMC plant biology, 19(1), 262-262 (2019-06-19)
Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed
Jimmy Su et al.
Journal of biomedical materials research. Part A, 106(9), 2448-2462 (2018-04-18)
Hydrogels, highly-hydrated crosslinked polymer networks, closely mimic the microenvironment of native extracellular matrix (ECM) and thus present as ideal platforms for three-dimensional cell culture. Hydrogels derived from tissue- and organ-specific decellularized ECM (dECM) may retain bioactive signaling cues from the
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Produkty

Proteinaza K: Wprowadzenie i zastosowania

Proteinaza K pomaga w zastosowaniach biologii molekularnej poprzez trawienie białek strukturalnych, usuwanie nukleaz i izolowanie nienaruszonego genomowego DNA.

Przewodnik dotyczący stosowania proteinazy K w różnych procedurach

Wytyczne dotyczące stosowania proteinazy K, enzymu powszechnie stosowanego do degradacji białek oraz ochrony DNA i RNA przed degradacją w próbkach.

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

Protokoły

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Protokół zestawu do miniprepcji genomowego DNA ssaków GenElute™

Protokół zestawu do oczyszczania genomowego DNA ssaków GenElute opisuje izolację czystego DNA o wysokiej masie cząsteczkowej z różnych źródeł ssaków.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

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