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Merck

10109118001

Roche

Odwrotna transkryptaza AMV

solution, >50 units/μg protein, suitable for RT-qPCR, suitable for RT-PCR

Synonim(y):

odwrotna transkryptaza amv

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About This Item

Numer EC enzymu:
Kod UNSPSC:
41105600

Formularz

solution

Poziom jakości

aktywność właściwa

>50 units/μg protein

Właściwości

dNTPs included: no
hotstart: no

opakowanie

pkg of 1,000 U

producent / nazwa handlowa

Roche

warunki przechowywania

avoid repeated freeze/thaw cycles

Parametry

42 °C optimum reaction temp.

metody

RT-PCR: suitable
RT-qPCR: suitable

moc wejściowa

purified RNA

metoda wykrywania

probe-based

Opis ogólny

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.

Specyficzność

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C

Zastosowanie

Reverse Transcriptase AMV is suitable for:
  • First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
  • Dideoxy DNA sequencing
  • Primer extension
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprint experiments

Cechy i korzyści

  • Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
  • Procure full length cDNA fragments up to 12 kb
  • Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

Opakowanie

1 kit containing 2 components

Jakość

Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.

Definicja jednostki

1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.

Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.

Uwaga dotycząca przygotowania

Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.

Przechowywanie i stabilność

Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
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Tylko elementy zestawu

Numer produktu
Opis

  • Reverse Transcriptase AMV 20-25 U/μl

  • First-strand cDNA Synthesis Buffer 5x concentrated

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


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