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P7059

Sigma-Aldrich

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonim(y):

Phosphate buffered saline, PBS

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About This Item

Numer MDL:
MFCD00131855
Kod UNSPSC:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

klasa czystości

for molecular biology

Poziom jakości

200

sterylność

0.2 μm filtered

Postać

liquid

metody

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

temp. przechowywania

room temp

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Opis ogólny

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Zastosowanie

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Komponenty

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Uwaga dotycząca przygotowania

Dilute 10x PBS to a 1x working solution using molecular biology grade water.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Imran Tarique et al.
Animals : an open access journal from MDPI, 9(11) (2019-11-07)
The ductuli efferentes (DE) form a transit passage for the passage of spermatozoa from the rete testis to the epididymis. After spermiation, various epithelial secretory proteins are transferred via extracellular vesicles (EVs) to the spermatozoa for their maturation and long-term
Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Caroline R Archer et al.
Scientific reports, 8(1), 10160-10160 (2018-07-07)
Pharmaceutical agents despite their efficacy to treat disease can cause additional unwanted cardiovascular side effects. Cardiotoxicity is characterized by changes in either the function and/or structure of the myocardium. Over recent years, functional cardiotoxicity has received much attention, however morphological
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