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O4387

Sigma-Aldrich

Oligo(dT)23, Anchored

70 μM in H2O

Synonim(y):

Oligo(dT)23 Anchored Primer

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About This Item

Kod UNSPSC:
41106305
NACRES:
NA.52

pochodzenie biologiczne

synthetic (organic)

Poziom jakości

200

Formularz

solution

zastosowanie

0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)

stężenie

70 μM in H2O

kolor

colorless

Warunki transportu

wet ice

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.

Zastosowanie

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Cechy i korzyści

  • In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
  • These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
  • Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
  • Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Inne uwagi

For laboratory use only. Not for drug, household or other uses.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCH1715
SLCD6862
SLCB2328
SLBW8572
SLBQ8955V

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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dNTP Set (10 mM each A,C,G,T) Cytiva 28-4065-64, pack of 500 μL (Total of 500 uL containing 10 mM each A,C,G,T)

GE28-4065-64

dNTP Set (10 mM each A,C,G,T)

Enhanced Avian HS RT-PCR Kit Flexible kit for one-step or two-step RT-PCR

Sigma-Aldrich

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Sigma-Aldrich

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Produkty

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

Odwrotna transkrypcja

Wyzwania w analizie ekspresji genów obejmują stabilność mRNA, czasowe wzorce transkrypcji i korelację mRNA-białko, co wpływa na dokładność.

Protokoły

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

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