About This Item
Kod UNSPSC:
41106609
NACRES:
NA.51
Polecane produkty
rekombinowane
expressed in E. coli
Postać
liquid
opakowanie
vial of 50 μL
stężenie
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
Zastosowanie
CRISPR
Promotor
Promoter name: CMV
selection
kanamycin
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
This product is an expression plasmid that utilizes the CMV promoter for strong transient expression of Dead Cas9 (CMV-dCas9). The dCas9 expression plasmid is one part of a two part CRISPR system with individual dCas9 and gRNA expression vectors.
To order gRNA in any format click here
To order gRNA in any format click here
Zastosowanie
Functional Genomics/Target Validation
- Proxy CRISPR
- Suitable for genomic DNA detection
Cechy i korzyści
- Highly specific
- Sequence verified
- Ready to use purified plasmid DNA
Zasada
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA).The Cas9 endonuclease can be rendered inactive (dCas9) with mutations to the two protein domains, RuvC and HnH (D10A and H840A respectively), which are responsible for nuclease activity. The nuclease deficient protein can then be programmed with a gRNA and directed to bind at a desired sequence of DNA. Variants of programmable endonucleases are often unable to cleave a large number of the targets that are efficiently cleaved by canonical SpCas9 in human cells. Cotargeting dead Cas9 may alter local chromatin structures and render otherwise inaccessible target sites now accessible and cleavable by alternative nucleases such as type II-B FnCas9, type II-C CjCas9 and NcCas9 and type V FnCpf1.
Informacje prawne
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Klienci oglądali również te produkty
Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting.
Chen, F. et al.
Nature Communications, 8 (2017)
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