HF1

94101908, rat liver, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: HF1, 94101908
• pochodzenie biologiczne: rat liver
• tryb wzrostu: Adherent
• kariotyp: Modal no. 100
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat hepatoma Hybrid [H5xFao]

Opis linii komórkowej

HF1 is a clone of hybrid cells resulting from the fusion of H5 and Fao. Expression of the majority of hepatic functions are extinguished. The cells contain a number of chromosomes corresponding to the sum of the parental numbers. HF1 variant which lacks the complete set of tissue-specific functions, appears to be a good candidate for regulatory mutants which control the orderly temporal expression of tissue specific functions during development.

Zastosowanie

Analysis of gene expression by molecular techniques.

pożywka hodowlana

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 9x1,000 to 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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