BRL-1

94121649, rat liver, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: BRL-1, 94121649
• pochodzenie biologiczne: rat liver
• tryb wzrostu: Adherent
• kariotyp: Diploid or pseudodiploid
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat epithelial cells

Opis linii komórkowej

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Zastosowanie

Feeder cells for co-culture with freshly isolated hepatocytes.

pożywka hodowlana

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Rutyna subkultury

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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