C2-Rev 7

94101907, rat liver, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: C2-Rev 7, 94101907
• pochodzenie biologiczne: rat liver
• tryb wzrostu: Adherent
• kariotyp: Hyperdiploid, 51 chromosomes
• morfologia: Epithelial
• produkty: Albumin and hepatocyte markers
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat hepatoma [HGPRT-]

Opis linii komórkowej

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

Zastosowanie

Gene expression studies.

pożywka hodowlana

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

Inne uwagi

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