MFE-280

98050131, human endometrium, Epithelial

• Synonim(y): MFE280 Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: MFE-280, 98050131
• pochodzenie biologiczne: human endometrium
• tryb wzrostu: Adherent
• kariotyp: Near diploid
• morfologia: Epithelial
• produkty: Placental protein 14
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human caucasian endometrial adenocarcinoma

Opis linii komórkowej

MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.

Zastosowanie

Tumour biology studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17

pożywka hodowlana

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Inne uwagi

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