Ishikawa Cell Line human
99040201, human uterus, Epithelial-like
Synonim(y):
ISHI Cells, Ishikawa Cells
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About This Item
Kod UNSPSC:
41106514
Polecane produkty
product name
Ishikawa Cell Line human, endometrial adenocarcinoma, 99040201
pochodzenie biologiczne
human uterus
tryb wzrostu
Adherent
kariotyp
Not specified
morfologia
Epithelial-like
produkty
Not specified
receptory
Oestrogen, progesterone
metody
cell culture | mammalian: suitable
powiązane choroby
cancer
Warunki transportu
dry ice
temp. przechowywania
−196°C
Pochodzenie linii komórkowej
Human Asian endometrial adenocarcinoma
Opis linii komórkowej
The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin. Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.
Zastosowanie
Ishikawa Cell Line human has been used to study the effect of 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3) on cytosolic pH regulation and glycolytic flux. It has also been used to study endometrial adenocarcinoma.
Profil DNA
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17
pożywka hodowlana
MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).
Rutyna subkultury
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; cells tend to pile up.
Inne uwagi
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