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C-12300

Sigma-Aldrich

Normal Human Dermal Fibroblasts (NHDF)

Juvenile foreskin, 500,000 cryopreserved cells

Synonim(y):

Human Dermal Fibroblast Cells, NHDF Cell Line

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About This Item

Kod UNSPSC:
41106514
NACRES:
NA.81

pochodzenie biologiczne

human foreskin (juvenile dermis)

opakowanie

pkg of 500,000 cells

morfologia

(fibroblast)

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

temp. przechowywania

−196°C

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Pochodzenie linii komórkowej

Skin

Zastosowanie

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Jakość

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Ostrzeżenie

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutyna subkultury

Click here for more information.

Inne uwagi

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Polecane produkty

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Produkty

Przegląd komórek pierwotnych i mediów wykorzystywanych w hodowlach biomedycznych podkreśla różnice w stosunku do linii komórkowych.

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

Pierwotne ludzkie fibroblasty i pożywka wzrostowa do hodowli in vitro młodych i dorosłych fibroblastów. Protokoły obsługi, pasażowania komórek, pożywki i stosowania produktu.

Protokoły

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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