106-05A
Human Dermal Fibroblasts: HDF, adult
Synonim(y):
HDF cells
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About This Item
Kod UNSPSC:
41106514
NACRES:
NA.81
Polecane produkty
pochodzenie biologiczne
human neonatal foreskin or adult skin (normal)
Poziom jakości
opakowanie
pkg of 500,000 cells
producent / nazwa handlowa
Cell Applications, Inc
tryb wzrostu
Adherent
kariotyp
2n = 46
morfologia
Fibroblast
metody
cell culture | mammalian: suitable
Warunki transportu
dry ice
temp. przechowywania
−196°C
Powiązane kategorie
Opis ogólny
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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Pochodzenie linii komórkowej
Skin
Zastosowanie
injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response
Komponenty
Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
Uwaga dotycząca przygotowania
- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
- Can be cultured at least 16 doublings.
pożywka hodowlana
Fibroblast Growth Medium (116-500) all-in-one ready to use media
Rutyna subkultury
Please refer to the HDF Culture Protocol.
Oświadczenie o zrzeczeniu się odpowiedzialności
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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wymagane, ale niedostarczone
Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Protokoły
Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
Niniejszy protokół zawiera: Przechowywanie - Przygotowanie do hodowli - Hodowla HDF - Podhodowla HDF
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