106-05A

Human Dermal Fibroblasts: HDF, adult

• Synonim(y): HDF cells
• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• pochodzenie biologiczne: human neonatal foreskin or adult skin (normal)
• Poziom jakości: 100
• opakowanie: pkg of 500,000 cells
• producent / nazwa handlowa: Cell Applications, Inc
• tryb wzrostu: Adherent
• kariotyp: 2n = 46
• morfologia: Fibroblast
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis ogólny

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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Pochodzenie linii komórkowej

Skin

Zastosowanie

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Komponenty

Fibroblast Basal Medium containing 10% FBS & 10% DMSO.

Uwaga dotycząca przygotowania

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
• Can be cultured at least 16 doublings.

pożywka hodowlana

Fibroblast Growth Medium (116-500) all-in-one ready to use media

Rutyna subkultury

Please refer to the HDF Culture Protocol.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable